Casper: LifeCanvas SmartBatch+

Electrophoretic Tissue Clearing and Immunolabeling

  • (Before using Casper) Protect tissue with SHIELD 
    • Preserve tissue architecture and fluorescent proteins with polyfunctional epoxide crosslinkers   Protocol
  • Delipidate quickly using Stochastic Electrotransport 
    • Disperse surfactant micelles throughout sample with rotational electric field   Protocol
  • Immunolabel with eFlash/SWITCH using Stochastic Electrotransport
    • Achieve uniform whole-organ labeling quickly by modulating antibody binding affinity - allow uniform antibody diffusion without surface target saturation   Protocol    Validated Antibodies
  • (After using Casper) Match Refractive Index with EasyIndex (RI 1.52)    Protocol

Please use your PPMS account to order samples and/or buffers for your clearing needs. In PPMS home page, navigate to "Order a service or consumable" and choose the appropriate service. Sample runs need to be a minimum of 4 samples.