Imaging Modality

  • Widefield
  • 2d Structured Illumination
  • 3D Structured Illumination
  • Structured Illumination/ Total Internal reflection
  • STED

SIM Hardware

  • Nikon Ti2 inverted microscope
  • N-SIM Spatial Light Modulator
  • Physik Instrument Piezo Z motor
  • Dual Hamamatsu Orca Flash 4.0 Camera
  • Nikon Laser Illumination: 405, 458, 514, 488, 561, 647
  • Widefield: DAPI, CFP, YFP, GFP, RFP
  • Nikon TIRF Lun-f Illumination: 488, 561, 640
  • Nikon Elements 5.11 acquisition Software

      • Abberior STED hardware

    • Abberior STED hardware
    • 405, 488 561 640 nm lasers
    • 775nm pulsed STED laser
    • Avalanche Photodiode detectors (APDs)

    Objectives

    • Plan Apo 20x/0.8 DIC M N2
    • SR HP Plan Apo λS 100x/1.4 Oil Silicon
    • SR HP Apo TIRF 100x/1.5 OIL  Automated Correction Collar

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