Imaging Modality

  • Single point scanning confocal
  • Super-resolution (Airyscan2)
  • Thick samples (fixed or alive)
  • DIC
  • Spectral Unmixing, FRAP, FCS

Hardware

  • Zeiss Axio Observer Z1
  • LSM980 Scan Head
  • Environmental enclosure
  • Motorized Stage
  • Sample Finder module
  • Zeiss Definite Focus 3
  • Laser lines: 405, 458, 488, 514, 561, 594, 639 and 730 nm
  • Detectors: Internal detectors: 2x multi-alkali PMT + GAaSP 32 Channel Spectral Detector (Spectral Imaging). External detectors: 2x NIR and Airyscan2 (super-resolution) 
  • X-Cite box Metal Halide illumination (widefield/locate sample)
  • DAPI, GFP and TexasRed filter cubes for widefield
  • Zen Blue Acquisition Software

Objectives

  • 2.5X - EC Plan Neo Fluor 2.5/0.085 Air DIC I
  • 10X - EC Plan NeoFluar 10x/0.3 Air DIC I
  • 25X - LD LCI Plan Apo 25x/0.8 Multi immersion DIC II
  • 32X - C Apo 32x/0.85 Water DIC III (swappable)
  • 40X - Plan Apo 40x/1.3 Oil DIC III
  • 63X - Plan Apo 63x/1.4 Oil DIC III

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