This microscope was purchased with a NIH S10 Grant. If used for your experiments, you must cite the specific S10 Grant: S10 RR027344-01
Imaging Modality
- Phase Contrast
- Objective heater for bacterial live cell imaging
- Widefield
- Total Internal Reflection
Hardware
- Nikon Ti inverted microscope
- Nikon motorized stage
- Andor Zyla 4.2 Plus sCMOS camera
- Andor iXon EM-CCD camera
- Lumencore SpectraX LED Illumination
- Nikon TIRF Lun-f Illumination: 488, 561, 640
- Chroma ET filter cubes in motorized filter turret: DAPI (49000), CFP (49001), GFP (49002), YFP (49003), and mCherry (49008)
- Nikon Elements 4.30 acquisition Software
Objectives
- Plan Apo 100x/1.4 Oil Ph3 DM
- Apo TIRF 100x/1.49 DIC N2
- Plan Fluor 40x/0.75 Ph2