This microscope was purchased with a NIH S10 Grant. If used for your experiments, you must cite the specific S10 Grant: S10 RR027344-01

 

Imaging Modality

 

  • Phase Contrast
  • Objective heater for bacterial live cell imaging
  • Widefield
  • Total Internal Reflection

Hardware

  • Nikon Ti inverted microscope
  • Nikon motorized stage
  • Andor Zyla 4.2 Plus sCMOS camera
  • Andor iXon EM-CCD camera
  • Lumencore SpectraX LED Illumination
  • Nikon TIRF Lun-f Illumination: 488, 561, 640
  • Chroma ET filter cubes in motorized filter turret: DAPI (49000), CFP (49001), GFP (49002), YFP (49003), and mCherry (49008)
  • Nikon Elements 4.30 acquisition Software

Objectives

  • Plan Apo 100x/1.4 Oil Ph3 DM
  • Apo TIRF 100x/1.49 DIC N2

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