Imaging Modality

  • Spinning disk confocal
  • Widefield
  • DIC
  • Long term live cell imaging

Hardware

  • Nikon Ti inverted microscope
  • W1 Yokogawa Spinning disk with 50 um pinhole disk
  • Laser lines: 405, 488, 561, 640
  • Filter cubes (for WIDEFIELD): DAPI (Chroma for 395 light source), GFP (Chroma ET 49002), mCherry (ET Chroma 49008), Cy5 (ET Chroma 49006)
  • Lumencor Sola LED Illumination for widefield
  • Nikon Motorized Stage
  • Physik Instrument Piezo Z motor
  • Andor Zyla 4.2 Plus sCMOS monochrome camera
  • OKO Lab Heated enclosure with CO2 control
  • Nikon Elements Acquisition Software AR 5.02

Objective Lenses

  • Plan Apo λD 10x/0.45 DIC
  • Plan Apo λ 20x/0.75 DIC I
  • S Plan Fluor 40x/1.3 Oil DIC H/N2
  • Plan Apo λD 60x/1.42 Oil DIC
  • Plan Apo λD 100x/1.45 Oil DIC
  • Apo LWD λS 40x/1.15 Oil DIC N2

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