Imaging Modality

  • Single point laser scanning confocal
  • Fixed, thick samples
  • DIC
  • Phase Contrast

Hardware

  • Olympus IX83
  • Multi-alkali PMT (x4) Spectral Imaging
  • prior Motorized stage
  • Laser lines: 405, 445, 488, 514, 561, and 640
  • Metal Halide for widefield illumination (locate sample)
  • DAPI, GFP, and Texas red filter cubes for widefield
  • FV31 SW Software for Acquisition

Objectives

  • Uplan S Apo 4x/0.16 Air
  • UPlan S Apo 10x/0.40 Air
  • UPlan X Apo 20x/0.8 Air
  • UPlan X Apo 40x/1.40 Oil
  • UPlan X Apo 60x/1.42 Oil

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