Imaging Modality
- Single point laser scanning confocal
- Multiphoton imaging
- FLIM (confocal and multiphoton)
- Multiplexing (>6 color imaging)
- Lightning (enhanced resolution)
- Fixed and Live thick samples
- Transmitted light
- Long term live imaging (full environmental enclosure with CO2 and active humidity)
Hardware
- Leica Upright DM6
- Stellaris 8 Falcon DIVA
- Motorized stage (Scientifica)
- Laser lines (confocal): 405 diode, WLL (440-790 nm tunable; 80Hz pulsed)
- Laser Lines (multiphoton): Insight X3 dual beam (tunable 690-1300nm and fixed at 1040 nm)
- Variable Beam Expander to adjust multiphoton excitation volume (optimize to improve resolution or depth)
- Confocal detectors: 2 HyDS, 1 HyDX and 1 HyDR. All spectral
- 4Tune Spectra non descanned spectral detection (multiphoton): 1 multi-alkali PMT, 2x HyD
- Full environmental enclosure for live imaging (with active humidity)
- LasX acquisition software
Objectives
- Plan Apo 10x/0.40 air
- Fluotar 16x/0.8 multi-immersion. Dipping. Correction collar from water to RI 1.57 (BABB)
- Plan Apo 20x multi-immersion (water with/without coverslip, glycerol, oil)
- Plan Apo 40x/0.8 water dipping
- Plan Apo 60x/0.9 water dipping
- Plan Apo 63X/1.4 Oil
- Plan Apo 25x/0.9 water dipping with motorized correction collar (single objective slider)