Hammerhead

Zeiss Lightsheet 7

Multi-view imaging in a variety of samples ranging from live organisms and organoids to fixed samples that have been optically cleared by both water-based (expansion, CLARITY, CUBIC, Scale) and solvent-based (iDisco)methods.

Hardware

Zeiss Lightsheet 7 with double side illumination 
With 37°C incubator and CO2

4 axis sample positioning system

Objectives

Lightsheet Illumination objectives 

  • 5x / NA-o.1 (for lower res illumination)
  • 10x / NA - 0.2 (for higher res illumination)

Detection objectives

  • 5x/0.16 Plan Neofluar (for overview images of large samples)
  • 10x/0.5 Plan Apochromat Water dipping (for live cell imaging of large samples or water-based-cleared samples)
  • 20x/1.0 Plan Apochromat Water dipping  (for high resolution live cell imaging)
  • 20x/1.0 Plan Apochromat with correction collar (for clearing solutions with RI of 1.45)
  • 20x/1.0 Plan Neofluar with correction collar (for clearing solutions with RI of 1.53)


Filters and dichroics

  • Quad dichroic 405/488/561/638
  • emission filter (dual emission)
    • DAPI (BP420-470) + GFP(BP505-545)
    • DAPI (BP420-470) + CY3 (BP575-615)
    • GFP (BP505-545) + mCherry, DRAQ5  (LP585)
    • GFP (BP505-545) + DRAQ5 (LP660)
    • Cy3 (BP575-615) + DRAQ5 (LP660)

Cameras

  • 2x PCO Edge 4.2 sCMOS - (6.5um pixel size)

Lasers

  • 405
  • 488
  • 561
  • 638