Hammerhead
Zeiss Lightsheet 7
Multi-view imaging in a variety of samples ranging from live organisms and organoids to fixed samples that have been optically cleared by both water-based (expansion, CLARITY, CUBIC, Scale) and solvent-based (iDisco)methods.
Hardware
Zeiss Lightsheet 7 with double side illumination
With 37°C incubator and CO2
4 axis sample positioning system
Objectives
Lightsheet Illumination objectives
- 5x / NA-o.1 (for lower res illumination)
- 10x / NA - 0.2 (for higher res illumination)
Detection objectives
- 5x/0.16 Plan Neofluar (for overview images of large samples)
- 10x/0.5 Plan Apochromat Water dipping (for live cell imaging of large samples or water-based-cleared samples)
- 20x/1.0 Plan Apochromat Water dipping (for high resolution live cell imaging)
- 20x/1.0 Plan Apochromat with correction collar (for clearing solutions with RI of 1.45)
- 20x/1.0 Plan Neofluar with correction collar (for clearing solutions with RI of 1.53)
Filters and dichroics
- Quad dichroic 405/488/561/638
- emission filter (dual emission)
- DAPI (BP420-470) + GFP(BP505-545)
- DAPI (BP420-470) + CY3 (BP575-615)
- GFP (BP505-545) + mCherry, DRAQ5 (LP585)
- GFP (BP505-545) + DRAQ5 (LP660)
- Cy3 (BP575-615) + DRAQ5 (LP660)
Cameras
- 2x PCO Edge 4.2 sCMOS - (6.5um pixel size)
Lasers
- 405
- 488
- 561
- 638