Hammerhead

Zeiss Lightsheet 7

Multi-view imaging in a variety of samples ranging from live organisms and organoids to fixed samples that have been optically cleared by both water-based (expansion, CLARITY, CUBIC, Scale) and solvent-based (iDisco)methods.

Hardware

Zeiss Lightsheet 7 with double side illumination 
For clared and live samples (37°C incubator and CO2)

Objectives

Lightsheet Illumination objectives 

• 5x / NA-o.1 (for lower res illumination)
• 10x / NA - 0.2 (for higher res illumination)

Detection objectives

• 5x/0.16 Plan Neofluar (for overview images of large samples)
• 10x/0.5 Plan Apochromat Water dipping (for live cell imaging of large samples or water-based-cleared samples)
• 20x/1.0 Plan Apochromat Water dipping  (for high resolution live cell imaging)
• 20x/1.0 Plan Apochromat with correction collar (for clearing solutions with RI of 1.45)
• 20x/1.0 Plan Neofluar with correction collar (for clearing solutions with RI of 1.53)

Filters and dichroics

• Quad dichroic 405/488/561/638
• emission filter (dual emission)
  • DAPI (BP420-470) + GFP(BP505-545)
  • DAPI (BP420-470) + CY3 (BP575-615)
  • GFP (BP505-545) + mCherry, DRAQ5  (LP585)
  • GFP (BP505-545) + DRAQ5 (LP660)
  • Cy3 (BP575-615) + DRAQ5 (LP660)

Cameras

• 2x PCO Edge 4.2 sCMOS - (6.5um pixel size)

Lasers

• 405
• 488
• 561
• 638