FLIMer
Imaging Modality
- Single point laser scanning confocal
- FLIM (fluorescence lifetime imaging - white light laser with pulse picker for capturing longer lifetimes)
- Multiplexing (>6 color imaging)
- Fixed and Live samples
- Transmitted light
- Live cell imaging (environmental enclosure with temperature control)
Hardware
- Leica Inverted DMi8
- Leica Stellaris 8 Falcon
- Motorized stage (ITK)
- Laser lines: 405 diode, WLL (440-790 tunable; pulse picker)
- Confocal detectors: 2 HydS, 2 HyDX, 1 HyDR, all spectral
- Full environmental enclosure for live imaging
- LasX acquisition software
Objectives
- Plan Apo 10x/0.40 Air
- Fluotar 16x/0.6 multi-immersion. Correction collar from water to RI 1.57
- Plan Apo 20x/0.75 Air
- Plan Apo 40x/1.25 Glycerol
- Plan Apo 63/1.40 Oil