Imaging Modality

  • Spinning disk confocal
  • Widefield
  • DIC
  • Long term live cell imaging

Hardware

  • Nikon Ti2 inverted microscope
  • W1 Yokogawa Spinning disk with 50 um pinhole disk
  • Laser lines: 405, 445, 488, 515, 561, 640
  • Digital Mirror Device (DMD) lines (Aura/LED): 350, 380, 488, 561
  • Emission Filters (for CONFOCAL): 455/50, 480/40, 525/36, 605/52, 630/75, 705/72
  • Filter cubes (for WIDEFIELD): DAPI (Chroma for 395 light source), GFP (Chroma ET 49002), mCherry (Chroma ET 49008), Cy5 (Chroma ET 49006)
  • Lumencor Sola LED Illumination for widefield
  • Nikon Motorized Stage
  • Physik Instrument Piezo Z motor
  • Andor Zyla 4.2 Plus sCMOS monochrome camera
  • OKO Lab Heated enclosure with CO2 control
  • Nikon Elements Acquisition Software AR 5.02

Objective Lenses

  • Plan Apo λ 20x/0.8 DIC I
  • Plan Fluor 40x/1.3 Oil DIC H/N2
  • APo λS LWD 40X/1.1 DICN2
  • Plan Apo λ 60x/1.4 Oil DIC
  • Plan Apo λ 100x/1.45 Oil DIC
  • Plan Apo λS SR HP 100xC/1.45 Silicon DIC

Request Access