Imaging Modality
- Spinning disk confocal
- Widefield
- DIC
- Long term live cell imaging
Hardware
- Nikon Ti2 inverted microscope
- W1 Yokogawa Spinning disk with 50 um pinhole disk
- Laser lines: 405, 445, 488, 515, 561, 640
- Digital Mirror Device (DMD) lines (Aura/LED): 350, 380, 488, 561
- Emission Filters (for CONFOCAL): 455/50, 480/40, 525/36, 605/52, 630/75, 705/72
- Filter cubes (for WIDEFIELD): DAPI (Chroma for 395 light source), GFP (Chroma ET 49002), mCherry (Chroma ET 49008), Cy5 (Chroma ET 49006)
- Lumencor Sola LED Illumination for widefield
- Nikon Motorized Stage
- Physik Instrument Piezo Z motor
- Andor Zyla 4.2 Plus sCMOS monochrome camera
- OKO Lab Heated enclosure with CO2 control
- Nikon Elements Acquisition Software AR 5.02
Objective Lenses
- Plan Apo λ 20x/0.8 DIC I
- Plan Fluor 40x/1.3 Oil DIC H/N2
- APo λS LWD 40X/1.1 DICN2
- Plan Apo λ 60x/1.4 Oil DIC
- Plan Apo λ 100x/1.45 Oil DIC
- Plan Apo λS SR HP 100xC/1.45 Silicon DIC