Imaging Modality
- Widefield
- DIC
- Phase Contrast
- Polarizing microscopy
- Color Imaging
Hardware
- Nikon Ti inverted microscope
- Prior ProScanIII motorized stage
- Nikon Z drive focus accessory
- Sutter Instrument Lambda 10-3 Motorized Excitation wheel. Filters: DAPI (Chroma AT350/50X), FITC(Chroma ET490/20X), TRITC (Chroma ET572/35X), CFP (Chroma ET 430/24), YFP (Chroma ET500/20X), Cy5 (Chroma ET 645/30X), Cy3 (Chroma ET 555/25x)
- Sutter Instrument LB101Q-219 Motorized Epifluorescence light path shutter
- Filter cubes: full set GFP cube (Chroma ET49002), Full Set Cy5 (Chroma ET49006), Multiband dichroic and excitation filter for DAPI/FITC/TexasRed (Chroma 69302), multiband dichroic and excitation filter for CFP/YFP (Chroma 59217), Multiband Dichroic for DAPI/Fitc/Cy3/Cy5 (Chroma 89100bs)
- Ludl Mac5000 PS System Motorized Emission Wheel. Filters: Cy5 (Chroma ET700/75M), Cy3 (Chroma ET605/52M), DAPI (Chroma ET455/50M), YFP (Chroma ET535/30M), ECFP/EYFP (Chroma ET470/24M)
- Lumencor SOLA LED light engine illumination
- Hamamatsu ORCA-Flash4.0 V3 Digital CMOS camera
- Nikon Elements Acquisition Software Version 5.21.03 build 1489
Objectives
- Plan Fluor 4x/0.13
- Plan Apo Lambda 10x/0.45 Air DIC N1
- Plan Apo 20x/0.75 Air Ph2 DM
- Plan Fluor 40X/1.30 Oil DIC N2
- Plan Apo Lambda 60x/1.40 Oil Ph3 DM
- Plan Apo Lambda 100x/1.45 Oil Ph3 DM