Imaging Modality
- Single point laser scanning confocal
- Multiphoton imaging
- FLIM (confocal and multiphoton)
- Multiplexing (>6 color imaging)
- Lightning (enhanced resolution)
- Fixed and Live thick samples
- Transmitted light
- Long term live imaging (full environmental enclosure with CO2 or 95% O2 and active humidity)
Objectives
- Plan Apo 10x/0.40 air
- Fluotar 16x/0.6 multi-immersion. Dipping. Correction collar from water to RI 1.57
Can be used with BABB, iDISCO, Water based media, Glycerol - Plan Apo 20x/0.75 multi-immersion
Can be used With/Without Coverslip & with Water, Glycerol, Oil - Plan Apo 40x/0.8
Water dipping only - Plan Apo 60x/0.9
Water dipping only - Plan Apo 63X/1.4
Oil only - Plan Apo 25x/0.9
Water dipping only with motorized correction collar (single objective slider)
Hardware
- Leica Upright DM6
- Stellaris 8 Falcon DIVA
- Motorized stage (Scientifica)
- Laser lines (confocal): 405 diode, WLL (440-790 nm tunable; 80Hz pulsed)
- Laser Lines (multiphoton): Insight X3 dual beam (tunable 690-1300nm and fixed at 1040 nm)
- Variable Beam Expander to adjust multiphoton excitation volume (optimize to improve resolution or depth)
- Confocal detectors: 2 HyDS, 1 HyDX and 1 HyDR. All spectral
- 4Tune Spectra non descanned spectral detection (multiphoton): 1 multi-alkali PMT, 2x HyD
- Full environmental enclosure for live imaging (with active humidity)
- LasX acquisition software